Anders Frisk

Anders Frisk

Molecular Microbiology Professional

Pelham, New York, United States
8 followers 8 connections

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Experience

  • Tulane University, Dept. of Microbiology and Immunology

    Tulane University, Dept. of Microbiology and Immunology

    9 years

    • Tulane University, Dept. of Microbiology and Immunology Graphic

      Research Instructor

      Tulane University, Dept. of Microbiology and Immunology

      - 4 years

      New Orleans

      Responsible for protein purification at core facility
      ~Purified target proteins by SEC, FPLC, affinity and ion-exchange chromatography
      ~Performed small and large-scale (10L) over-expression of native and recombinant target proteins
      ~Purified recombinant His-tagged LT toxin A-subunit of E. coli for antibody production
      ~Redesigned purification strategy and improved purity/yield of a Y. pestis F1-V fusion protein for vaccine studies

    • Tulane University, Dept. of Microbiology and Immunology Graphic

      Postdoctoral Fellow

      Tulane University, Dept. of Microbiology and Immunology

      - 5 years

      New Orleans

      Research was focused on the pathogenesis of Pseudomonas aeruginosa, mostly on the transcriptional regulator AlgR
      ~Investigated AlgR regulated genes and the regulation of the algR gene itself
      ~Designed primers, generated gene targets with PCR, performed cloning in E. coli expression plasmids and transcriptional promoter fusion plasmids
      ~Generated in-frame deletions or replacements in bacterial chromosomal gene targets
      ~Examined 32P-labelled protein-DNA interactions by gel mobility…

      Research was focused on the pathogenesis of Pseudomonas aeruginosa, mostly on the transcriptional regulator AlgR
      ~Investigated AlgR regulated genes and the regulation of the algR gene itself
      ~Designed primers, generated gene targets with PCR, performed cloning in E. coli expression plasmids and transcriptional promoter fusion plasmids
      ~Generated in-frame deletions or replacements in bacterial chromosomal gene targets
      ~Examined 32P-labelled protein-DNA interactions by gel mobility shift assays
      ~Determined by microarrays the transcriptional profile of P. aeruginosa genes following interaction with host cells

  • University of Florida, Dept. of Medicine Graphic

    Postdoctoral Fellow

    University of Florida, Dept. of Medicine

    - 2 years

    Gainesville, Florida

    ~Identified a flagellin regulator gene (flgM)
    ~Generated a chromosomal mutation in the flgM gene
    ~Optimized a two-hybrid yeast transcriptional assay to assess in vivo protein-protein interactions

  • University of Gothenburg Graphic

    Research Associate

    University of Gothenburg

    - 1 year

    Gothenburg, Sweden

    ~Constructed plasmids expressing recombinant H. ducreyi multi-component cytotoxin
    ~Performed cell culture cytotoxicity and cytotoxin neutralization assays

Education

  • University of Gothenburg Graphic

    University of Gothenburg

    Doctor of Philosophy (Ph.D.) Medical Microbiology

    Activities and Societies: Journal Club

    Surface antigens of Haemophilus ducreyi; Characterization, localization, role in adherence and mechanism of host defense.
    Techniques used:
    ~Protein isolation and purification (ion exchange and size exclusion chromatography)
    ~Production of anti-protein rabbit serum
    ~Cell-culture, SDS-PAGE, Western blot, ELISA, Transmission electron microscopy

  • Lund University Graphic

    Lund University

    Bachelor of Science (B.S.) Chemistry

Publications

Courses

  • Bioinformatics, Microarray Analysis, Genespring, Cystic Fibrosis Foundation Therapeutics Inc.

    -

Languages

  • English

    Professional working proficiency

  • Swedish

    Native or bilingual proficiency

  • Persian

    Elementary proficiency

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